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anti p2x3r rabbit  (Alomone Labs)


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    Structured Review

    Alomone Labs anti p2x3r rabbit
    Anti P2x3r Rabbit, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p2x3/Anti-P2X3+Receptor+Antibody/pm42158953-102-12-17
    Average 94 stars, based on 36 article reviews
    anti p2x3r rabbit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Amplified P2X<sub>3</sub> pathway activity in muscle afferent dorsal root ganglion neurons and exercise pressor reflex regulation in hindlimb ischaemia–reperfusion
    Article Snippet: Thirty micrograms of protein was loaded in 10% Mini-Protean TGX Precast gels (Bio-Rad Laboratories, Hercules, CA, USA) after being boiled at 95◦C for 5min in SDS sample buffer, then electrophoretically transferred to polyvinylidene difluoride membrane. .. After blocking with 5% non-fat milk in 0.1% Tween–Tris-buffered saline (TBST) for 1 h, the membrane was incubated with rabbit anti-P2X3 (1:1000, Alomone Laboratories, Jerusalem, Israel) primary antibody at 4◦C overnight and then incubatedwith horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:2000, Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. Immunoreactivity was visualized using an enhanced chemiluminescence system (Cell Signaling Technology). .. Themembranewas stripped and incubatedwith anti-β-actin primary antibody (1:1000, Sigma-Aldrich, St Louis, MO, USA) as the internal protein expression control.

    Saline:

    Article Title: Amplified P2X<sub>3</sub> pathway activity in muscle afferent dorsal root ganglion neurons and exercise pressor reflex regulation in hindlimb ischaemia–reperfusion
    Article Snippet: Thirty micrograms of protein was loaded in 10% Mini-Protean TGX Precast gels (Bio-Rad Laboratories, Hercules, CA, USA) after being boiled at 95◦C for 5min in SDS sample buffer, then electrophoretically transferred to polyvinylidene difluoride membrane. .. After blocking with 5% non-fat milk in 0.1% Tween–Tris-buffered saline (TBST) for 1 h, the membrane was incubated with rabbit anti-P2X3 (1:1000, Alomone Laboratories, Jerusalem, Israel) primary antibody at 4◦C overnight and then incubatedwith horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:2000, Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. Immunoreactivity was visualized using an enhanced chemiluminescence system (Cell Signaling Technology). .. Themembranewas stripped and incubatedwith anti-β-actin primary antibody (1:1000, Sigma-Aldrich, St Louis, MO, USA) as the internal protein expression control.

    Membrane:

    Article Title: Amplified P2X<sub>3</sub> pathway activity in muscle afferent dorsal root ganglion neurons and exercise pressor reflex regulation in hindlimb ischaemia–reperfusion
    Article Snippet: Thirty micrograms of protein was loaded in 10% Mini-Protean TGX Precast gels (Bio-Rad Laboratories, Hercules, CA, USA) after being boiled at 95◦C for 5min in SDS sample buffer, then electrophoretically transferred to polyvinylidene difluoride membrane. .. After blocking with 5% non-fat milk in 0.1% Tween–Tris-buffered saline (TBST) for 1 h, the membrane was incubated with rabbit anti-P2X3 (1:1000, Alomone Laboratories, Jerusalem, Israel) primary antibody at 4◦C overnight and then incubatedwith horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:2000, Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. Immunoreactivity was visualized using an enhanced chemiluminescence system (Cell Signaling Technology). .. Themembranewas stripped and incubatedwith anti-β-actin primary antibody (1:1000, Sigma-Aldrich, St Louis, MO, USA) as the internal protein expression control.

    Incubation:

    Article Title: Amplified P2X<sub>3</sub> pathway activity in muscle afferent dorsal root ganglion neurons and exercise pressor reflex regulation in hindlimb ischaemia–reperfusion
    Article Snippet: Thirty micrograms of protein was loaded in 10% Mini-Protean TGX Precast gels (Bio-Rad Laboratories, Hercules, CA, USA) after being boiled at 95◦C for 5min in SDS sample buffer, then electrophoretically transferred to polyvinylidene difluoride membrane. .. After blocking with 5% non-fat milk in 0.1% Tween–Tris-buffered saline (TBST) for 1 h, the membrane was incubated with rabbit anti-P2X3 (1:1000, Alomone Laboratories, Jerusalem, Israel) primary antibody at 4◦C overnight and then incubatedwith horseradish peroxidase-conjugated anti-rabbit secondary antibody (1:2000, Cell Signaling Technology, Danvers, MA, USA) at room temperature for 1 h. Immunoreactivity was visualized using an enhanced chemiluminescence system (Cell Signaling Technology). .. Themembranewas stripped and incubatedwith anti-β-actin primary antibody (1:1000, Sigma-Aldrich, St Louis, MO, USA) as the internal protein expression control.



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